Cell Signaling Phospho-Fak (Tyr397) Antibody

Name: Cell Signaling Phospho-Fak (Tyr397) Antibody
SKU: CSSPQ-3283S
Price: 362.00 USD

List Price $362.00 Your Price $362.00

Cell Signaling Phospho-Fak (Tyr397) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-3283S

MFG.PART: 3283S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Hm Pg
Sensitivity	Endogenous
Molecular Weight (kDa)	125
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs.
Species Reactivity	Human, Mouse, Rat, Hamster, Pig
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).