Cell Signaling Zeb1 (D80d3) Rabbit mAb

Name: Cell Signaling Zeb1 (D80d3) Rabbit mAb
SKU: CSSPQ-3396T
Price: 152.00 USD

List Price $152.00 Your Price $152.00

Cell Signaling Zeb1 (D80d3) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-3396T

MFG.PART: 3396T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	200
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	TCF8/ZEB1 (D80D3) Rabbit mAb detects endogenous levels of total TCF8/ZEB1 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region around residue Asp868 of human TCF8/ZEB1.
Background	ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. There are two members in mammals, ZEB1 (δ-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). One of the targets suppressed by ZEB proteins is E-cadherin. Downregulation of E-cadherin is one of the hallmarks of epithelial mesenchymal transition (EMT), a critical feature of normal embryonic development, which is also utilized by malignant epithelial tumors to spread beyond their origin (2-4). ZEB1 mutations are associated with posterior corneal dystrophy, and ZEB2 mutations were reported to be associated with Hirschsprung (HSCR) disease (5-8).