Cell Signaling Pdi (C81h6) Rabbit mAb

Name: Cell Signaling Pdi (C81h6) Rabbit mAb
SKU: CSSPQ-3501T
Price: 149.00 USD

List Price $149.00 Your Price $149.00

Cell Signaling Pdi (C81h6) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-3501T

MFG.PART: 3501T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	57
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:1250, Immunofluorescence (Immunocytochemistry): 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PDI (C81H6) Rabbit mAb detects endogenous levels of total PDI protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDI.
Background	During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To REACH their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).