Cell Signaling Phospho-Lat (Tyr220) Antibody

Name: Cell Signaling Phospho-Lat (Tyr220) Antibody
SKU: CSSPQ-3584T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Phospho-Lat (Tyr220) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-3584T

MFG.PART: 3584T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	36, 38
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho LAT (Tyr220) Antibody detects endogenous levels of LAT only when phosphorylated at tyrosine 220. Tyr220 of LAT, long isoform corresponds to Tyr191 of LAT, short isoform.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr220 of human LAT. Antibodies are purified by protein A and peptide affinity chromatography.
Background	LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).