We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Cell Signaling Frizzled5 Antibody
List Price
$394.00
Your Price
$394.00
Cell Signaling Frizzled5 Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-3795S
MFG.PART:
3795S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Transfected Only |
| Molecular Weight (kDa) | 50-65 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Frizzled5 Antibody detects transfected levels of total frizzled5 protein. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to the amino acid sequence surrounding Ala549 of human frizzled5. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Frizzled (Fzd) belongs to the seven transmembrane-spanning g-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11). |
| SKU | CSSPQ-3795S |
|---|---|
| Supplier Part Number | 3795S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |