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Cell Signaling Phospho-Beta-Catenin (Ser675) (D2f1) Xp Rabbit mAb
List Price
$179.00
Your Price
$179.00
Cell Signaling Phospho-Beta-Catenin (Ser675) (D2f1) Xp Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-4176T
MFG.PART:
4176T
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 20 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 92 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:200, Immunofluorescence (Frozen): 1:100, Immunofluorescence (Immunocytochemistry): 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb detects endogenous levels of β-catenin only when phosphorylated at Ser675. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin. |
| Background | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9,10). |
| SKU | CSSPQ-4176T |
|---|---|
| Supplier Part Number | 4176T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |