Cell Signaling Helios (D8w4x) Xp Rabbit mAb

Name: Cell Signaling Helios (D8w4x) Xp Rabbit mAb
SKU: CSSPQ-42427T
Price: 161.00 USD

List Price $161.00 Your Price $161.00

Cell Signaling Helios (D8w4x) Xp Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-42427T

MFG.PART: 42427T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	58, 63-70, 85
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:200, Flow Cytometry (Fixed/Permeabilized): 1:100, Chromatin IP: 1:50, Chromatin IP-seq: 1:50, CUT&RUN: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Helios (D8W4X) XP® Rabbit mAb recognizes endogenous levels of total Helios protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly73 of human Helios protein.
Background	Helios (Ikaros family zinc finger 2, IKZF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IKZF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).