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Cell Signaling Phospho-P38 Mapk (Thr180/Tyr182) (12f8) Rabbit mAb
List Price
$412.00
Your Price
$412.00
Cell Signaling Phospho-P38 Mapk (Thr180/Tyr182) (12f8) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-4631S
MFG.PART:
4631S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 200 µl |
| Reactivity | H M R Mk Dm |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 43 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:100, Immunofluorescence (Immunocytochemistry): 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit mAb detects endogenous levels of p38 MAPK only when dually phosphorylated at threonine 180 and tyrosine182. It will also react with p38 singly phosphorlyated at Thr180. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. |
| Species Reactivity | Human, Mouse, Rat, Monkey, D. melanogaster |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. |
| Background | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| SKU | CSSPQ-4631S |
|---|---|
| Supplier Part Number | 4631S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |