Cell Signaling Cox IV Antibody

Name: Cell Signaling Cox IV Antibody
SKU: CSSPQ-4844S
Price: 314.00 USD

List Price $314.00 Your Price $314.00

Cell Signaling Cox IV Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-4844S

MFG.PART: 4844S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk B
Sensitivity	Endogenous
Molecular Weight (kDa)	17
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:2400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	COX IV Antibody detects endogenous levels of total COX IV protein.
Species Reactivity	Human, Mouse, Rat, Monkey, Bovine
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Background	Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.

Specifications