Cell Signaling Phospho-Cpla2 (Ser505) (D4i2a) Rabbit mAb

Name: Cell Signaling Phospho-Cpla2 (Ser505) (D4i2a) Rabbit mAb
SKU: CSSPQ-53044S
Price: 415.00 USD

List Price $415.00 Your Price $415.00

Cell Signaling Phospho-Cpla2 (Ser505) (D4i2a) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-53044S

MFG.PART: 53044S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	95
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-cPLA2 (Ser505) (D4I2A) Rabbit mAb recognizes endogenous levels of cPLA2 protein only when phosphorylated at Ser505.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser505 of human cPLA2 protein.
Background	Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.