Cell Signaling Cleaved Parp (Asp214) (D6x6x) Rabbit mAb (Rodent Specific) (PE Conjugate)

Name: Cell Signaling Cleaved Parp (Asp214) (D6x6x) Rabbit mAb (Rodent Specific) (PE Conjugate)
SKU: CSSPQ-67495S
Price: 493.00 USD

List Price $493.00 Your Price $493.00

Cell Signaling Cleaved Parp (Asp214) (D6x6x) Rabbit mAb (Rodent Specific) (PE Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-67495S

MFG.PART: 67495S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	M R
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Cleaved PARP (Asp214) (D6X6X) Rabbit mAb (PE Conjugate) recognizes endogenous levels of the large fragment (89 kDa) of rodent PARP protein only when cleaved at Asp214.
Species Reactivity	Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of rodent PARP1 protein.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Specifications