Cell Signaling Smad2/3 (D7g7) Xp Rabbit mAb (PE Conjugate)

Name: Cell Signaling Smad2/3 (D7g7) Xp Rabbit mAb (PE Conjugate)
SKU: CSSPQ-72255S
Price: 465.00 USD

List Price $465.00 Your Price $465.00

Cell Signaling Smad2/3 (D7g7) Xp Rabbit mAb (PE Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-72255S

MFG.PART: 72255S

UNSPSC: 41116111

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	SMAD2/3 (D7G7) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total SMAD2/3 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His198 of human SMAD2/3 protein.
Background	Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).