Cell Signaling Cleaved Caspase-9 (Asp330) (D2d4) Rabbit mAb

Name: Cell Signaling Cleaved Caspase-9 (Asp330) (D2d4) Rabbit mAb
SKU: CSSPQ-7237T
Price: 169.00 USD

List Price $169.00 Your Price $169.00

Cell Signaling Cleaved Caspase-9 (Asp330) (D2d4) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-7237T

MFG.PART: 7237T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	37
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330. Full-length caspase-9 may be weakly detected with some cell lines.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp330 of human caspase-9 protein.
Background	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).