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Cell Signaling Phospho-Pzr (Tyr263) (D6a5) Rabbit mAb
List Price $415.00 Your Price $415.00
Cell Signaling Phospho-Pzr (Tyr263) (D6a5) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-8088S
MFG.PART: 8088S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R B |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 30-50 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:400, Flow Cytometry (Fixed/Permeabilized): 1:6400} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-PZR (Tyr263) (D6A5) Rabbit mAb recognizes endogenous levels of PZR protein only when phosphorylated at Tyr263. |
| Species Reactivity | Human, Mouse, Rat, Bovine |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr263 of human PZR protein. |
| Background | PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9). |
| SKU | CSSPQ-8088S |
|---|---|
| Supplier Part Number | 8088S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |