Cell Signaling Apaf-1 (D5c3) Rabbit mAb

Name: Cell Signaling Apaf-1 (D5c3) Rabbit mAb
SKU: CSSPQ-8969T
Price: 149.00 USD

List Price $149.00 Your Price $149.00

Cell Signaling Apaf-1 (D5c3) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-8969T

MFG.PART: 8969T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	135
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Apaf-1 (D5C3) Rabbit mAb recognizes endogenous levels of total Apaf-1 protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro470 of human Apaf-1 protein.
Background	Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).