Cell Signaling Cdc2 (Poh1) Mouse mAb

Name: Cell Signaling Cdc2 (Poh1) Mouse mAb
SKU: CSSPQ-9116T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Cdc2 (Poh1) Mouse mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9116T

MFG.PART: 9116T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	34
Source/Isotype	Mouse IgG2a
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	cdc2 (POH1) Mouse mAb detects endogenous levels of total cdc2 protein. The antibody does not cross-react with other cdks.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein. This antibody recognizes residues near the carboxy-terminus of human cdc2.
Background	The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).