Cell Signaling Mek2 (13e3) Rabbit mAb

Name: Cell Signaling Mek2 (13e3) Rabbit mAb
SKU: CSSPQ-9147S
Price: 348.00 USD

List Price $348.00 Your Price $348.00

Cell Signaling Mek2 (13e3) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9147S

MFG.PART: 9147S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MEK2 (13E3) Rabbit mAb detects endogenous levels of total MEK2 protein. This antibody does not cross-react with MEK1 and other MAP kinase kinases.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human MEK2.
Background	MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.