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Cell Signaling Phospho-P38 Mapk (Thr180/Tyr182) Antibody
List Price $362.00 Your Price $362.00
Cell Signaling Phospho-P38 Mapk (Thr180/Tyr182) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-9211S
MFG.PART: 9211S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 200 µl |
| Reactivity | H M R Mk Dm Pg Sc |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 43 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:400} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-p38 MAPK (Thr180/Tyr182) Antibody detects endogenous levels of p38 MAPK only when activated by phosphorylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. |
| Species Reactivity | Human, Mouse, Rat, Monkey, D. melanogaster, Pig, S. cerevisiae |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAPK. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| SKU | CSSPQ-9211S |
|---|---|
| Supplier Part Number | 9211S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |