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Cell Signaling Sapk/Jnk Antibody
List Price $135.00 Your Price $135.00
Cell Signaling Sapk/Jnk Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-9252T
MFG.PART: 9252T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H M R Hm Mk Z B Sc |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 46, 54 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | SAPK/JNK Antibody detects endogenous levels of total JNK1, JNK2 or JNK3 protein. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Zebrafish, Bovine, S. cerevisiae |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a recombinant human JNK2 fusion protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| SKU | CSSPQ-9252T |
|---|---|
| Supplier Part Number | 9252T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |