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Cell Signaling Phospho-Bad (Ser155) Antibody
List Price $148.00 Your Price $148.00
Cell Signaling Phospho-Bad (Ser155) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-9297T
MFG.PART: 9297T
UNSPSC: 12352202
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | M |
| Sensitivity | Transfected Only |
| Molecular Weight (kDa) | 23 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-Bad (Ser155) Antibody detects transfected levels of Bad only when phosphorylated at Ser155. This antibody does not detect Bad phosphorylated at other sites or related family members. |
| Species Reactivity | Mouse |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser155 of mouse Bad. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). |
| SKU | CSSPQ-9297T |
|---|---|
| Supplier Part Number | 9297T |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |