Cell Signaling Cleaved Caspase-7 (Asp198) Antibody

Name: Cell Signaling Cleaved Caspase-7 (Asp198) Antibody
SKU: CSSPQ-9491T
Price: 182.00 USD

List Price $182.00 Your Price $182.00

Cell Signaling Cleaved Caspase-7 (Asp198) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9491T

MFG.PART: 9491T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	20
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-7 (Asp198) Antibody detects endogenous levels of the large fragment of caspase-7 resulting from cleavage at aspartic acid 198. The antibody does not cross-react with full length caspase-7 or with other caspases.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp198 in human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

Specifications