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Cell Signaling Caspase-9 Antibody (Mouse Specific)
List Price $135.00 Your Price $135.00
Cell Signaling Caspase-9 Antibody (Mouse Specific) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-9504T
MFG.PART: 9504T
UNSPSC: 12352202
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 37, 39, 49 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Caspase-9 Antibody detects endogenous levels of both full length mouse caspase-9 (49 kDa) and the large fragment of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). The antibody does not cross-react other caspases. |
| Species Reactivity | Mouse |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). |
| SKU | CSSPQ-9504T |
|---|---|
| Supplier Part Number | 9504T |
| UM | EA |
| UNSPSC | 12352202 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |