Cell Signaling Phospho-Cdc25c (Ser216) Antibody

Name: Cell Signaling Phospho-Cdc25c (Ser216) Antibody
SKU: CSSPQ-9528S
Price: 427.00 USD

List Price $427.00 Your Price $427.00

Cell Signaling Phospho-Cdc25c (Ser216) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9528S

MFG.PART: 9528S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	60
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-cdc25C (Ser216) Antibody detects endogenous levels of cdc25C only when phosphorylated at Ser216.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).