Cell Signaling Cleaved Parp (Asp214) Antibody (Rat Specific)

Name: Cell Signaling Cleaved Parp (Asp214) Antibody (Rat Specific)
SKU: CSSPQ-9545T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Cleaved Parp (Asp214) Antibody (Rat Specific) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9545T

MFG.PART: 9545T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	R
Sensitivity	Endogenous
Molecular Weight (kDa)	89
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved PARP (Asp214) Antibody detects endogenous levels of the large fragment of PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Species Reactivity	Rat
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in rat PARP. Antibodies are purified by protein A and peptide affinity chromatography.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)