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Cell Signaling Phospho-Smad1/5 (Ser463/465) (41d10) Rabbit mAb (Biotinylated)
List Price $493.00 Your Price $493.00
Cell Signaling Phospho-Smad1/5 (Ser463/465) (41d10) Rabbit mAb (Biotinylated) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-9576S
MFG.PART: 9576S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 136 mm NaCl, 2.6 mm KCI, 12 mm sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
| Specificity/Sensitivity | Phospho-SMAD1/5 (Ser463/465) (41D10) Rabbit mAb (Biotinylated) detects endogenous levels of SMAD1/5 only when dually phosphorylated at Ser463 and Ser465. The antibody does not cross-react with other SMAD-related proteins. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD5. |
| Background | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β superfamily of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate SMAD1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as SMAD5 and SMAD9 (SMAD8) at their corresponding sites. These phosphorylated SMADs dimerize with the coactivating SMAD4 and translocate to the nucleus, where they regulate the transcription of target genes (5). MAP kinases and CDKs 8 and 9 are also reported to phosphorylate residues in the linker region of SMAD1, including Ser206. Phosphorylation of SMAD1 at Ser206 recruits Smurf1 to the linker region and leads to the degradation of SMAD1 (6). Phosphorylation at this site also promotes SMAD1 transcriptional activity by recruiting YAP to the linker region (7). |
| SKU | CSSPQ-9576S |
|---|---|
| Supplier Part Number | 9576S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |