Cell Signaling Beta-Catenin Antibody (Carboxy-Terminal Antigen)

Name: Cell Signaling Beta-Catenin Antibody (Carboxy-Terminal Antigen)
SKU: CSSPQ-9587T
Price: 135.00 USD

List Price $135.00 Your Price $135.00

Cell Signaling Beta-Catenin Antibody (Carboxy-Terminal Antigen) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9587T

MFG.PART: 9587T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	92
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:800, Chromatin IP: 1:25}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).