Cell Signaling Phospho-Msk1 (Thr581) Antibody

Name: Cell Signaling Phospho-Msk1 (Thr581) Antibody
SKU: CSSPQ-9595T
Price: 148.00 USD

List Price $148.00 Your Price $148.00

Cell Signaling Phospho-Msk1 (Thr581) Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9595T

MFG.PART: 9595T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	90
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-MSK1 (Thr581) Antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581. This antibody does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3.
Species Reactivity	Human, Mouse
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.
Background	MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.