Cell Signaling Caspase-8 (1c12) Mouse mAb

Name: Cell Signaling Caspase-8 (1c12) Mouse mAb
SKU: CSSPQ-9746T
Price: 147.00 USD

List Price $147.00 Your Price $147.00

Cell Signaling Caspase-8 (1c12) Mouse mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-9746T

MFG.PART: 9746T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	18, 43, 57
Source/Isotype	Mouse IgG1
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Caspase-8 (1C12) Mouse mAb detects endogenous levels of full length caspase-8 (57 kDa), the cleaved intermediate p43/p41 and the caspase-8 active fragment p18. This antibody does not cross-react with other caspases.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of the p18 fragment of human caspase-8. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.
Background	Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.