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Cell Signaling Cd16/Cd32 (2.4g2) Rat mAb
List Price $106.00 Your Price $106.00
Cell Signaling Cd16/Cd32 (2.4g2) Rat mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-88280S
MFG.PART: 88280S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µg |
| Reactivity | M |
| Sensitivity | Endogenous |
| Source/Isotype | Rat IgG2b |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200} |
| Storage | Supplied in 10 mm NaH2PO4, 150 mm NaCl, 0.09% NaN3, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze. |
| Specificity/Sensitivity | CD16/CD32 (2.4G2) Rat mAb recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32. |
| Species Reactivity | Mouse |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. |
| Background | CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).The 2.4G2 clone is widely used as a pre-treatment reagent to reduce non-specific binding of mouse Fc-receptors (4). |
| SKU | CSSPQ-88280S |
|---|---|
| Supplier Part Number | 88280S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |