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Cell Signaling Yap/Taz (D24e4) Rabbit mAb (Alexa Fluor 647 Conjugate)
List Price
$465.00
Your Price
$465.00
Cell Signaling Yap/Taz (D24e4) Rabbit mAb (Alexa Fluor 647 Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-10179S
MFG.PART:
10179S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M Mk |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | YAP/TAZ (D24E4) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total YAP and TAZ proteins. |
| Species Reactivity | Human, Mouse, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp362 of human TAZ protein. |
| Background | YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size (6-8). Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (10).TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins (11). TAZ shares homology with the WW domain of Yes-associated protein (YAP) (11). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (12,13). TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development (14). |
| SKU | CSSPQ-10179S |
|---|---|
| Supplier Part Number | 10179S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |