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Cell Signaling Sars-Cov-2 3’-5’ Exonuclease Antibody
List Price $317.00 Your Price $317.00
Cell Signaling Sars-Cov-2 3’-5’ Exonuclease Antibody - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-99098S
MFG.PART: 99098S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | Vir |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | SARS-CoV-2 3-5 Exonuclease Antibody recognizes endogenous levels of total SARS-CoV-2 3-5 exonuclease protein. |
| Species Reactivity | Virus |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val125 of SARS-CoV-2 3-5 exonuclease protein. Antibodies are purified by peptide affinity chromatography. |
| Background | The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2).SARS-CoV-2 3’-5’ exonuclease, or ExoN, is a non-structural protein (Nsp14) translated from ORF1b (2). It is a proofreading enzyme that functions in the replicase holoenzyme to read, excise, and repair errors in the newly transcribed viral RNA (3). RNA viruses generally have a high mutation rate and Coronaviridae, and others among the Nidovirales genus, have the largest and most complex genomes of RNA viruses (4). The 3’-5’ exonuclease is highly conserved between Coronaviruses, suggesting it’s important to the maintenance of such a large genome (5). The 3’-5’ exonuclease also provides a functionality to the RNA capping process, alongside the 2’-ribose methyl transferase (Nsp14), the helicase (Nsp13), and a guanylyl transferase (6,7). It is, however, the exonuclease function that is the key challenge to antiviral drug design with nucleoside analogue approaches (8). |
| SKU | CSSPQ-99098S |
|---|---|
| Supplier Part Number | 99098S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |