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Cell Signaling Phospho-Tau (Thr231/Ser235) (E7g5w) Rabbit mAb
List Price $415.00 Your Price $415.00
Cell Signaling Phospho-Tau (Thr231/Ser235) (E7g5w) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-20473S
MFG.PART: 20473S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H M R |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 55-80 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-Tau (Thr231/Ser235) (E7G5W) Rabbit mAb recognizes endogenous levels of tau protein only when phosphorylated at Thr231 and Thr231/Ser235. This antibody does not detect phosphorylation at Ser235 alone. |
| Species Reactivity | Human, Mouse, Rat |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr231 of human tau protein. |
| Background | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimers disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).It has been shown that phosphorylation of tau at Thr231/Ser235 is elevated in AD brain and human tau-transgenic mouse models of AD, where it may be involved in increasing tau seeding efficiency (4,5). Tau phosphorylation at Thr231 can be detected in cerebrospinal fluid and plasma and shows potential as a biomarker of neurofibrillary pathology in AD (6,7). |
| SKU | CSSPQ-20473S |
|---|---|
| Supplier Part Number | 20473S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |