Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (BSA And Azide Free)

Name: Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (BSA And Azide Free)
SKU: CSSPQ-39788SF
Price: 898.00 USD

List Price $898.00 Your Price $898.00

Cell Signaling Phospho-4e-Bp1 (Thr37/46) (236b4) Rabbit mAb (BSA And Azide Free) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-39788SF

MFG.PART: 39788SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µg
Reactivity	H M R Mk Dm
Sensitivity	Endogenous
Molecular Weight (kDa)	15 to 20
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #2855.
Specificity/Sensitivity	Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (BSA and Azide Free) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.
Species Reactivity	Human, Mouse, Rat, Monkey, D. melanogaster
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.
Background	Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).