Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (BSA And Azide Free)

Name: Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (BSA And Azide Free)
SKU: CSSPQ-95696SF
Price: 898.00 USD

List Price $898.00 Your Price $898.00

Cell Signaling Cleaved Parp (Asp214) (D64e10) Rabbit mAb (BSA And Azide Free) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-95696SF

MFG.PART: 95696SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µg
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	89
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #5625.
Specificity/Sensitivity	Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. The antibody does not recognize full-length PARP1 or other PARP isoforms.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 in human PARP.
Background	PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Specifications