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Cell Signaling Cleaved Caspase-3 (Asp175) (5a1e) Rabbit mAb (Alexa Fluor ® 488 Conjugate)
List Price $493.00 Your Price $493.00
Cell Signaling Cleaved Caspase-3 (Asp175) (5a1e) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-67308S
MFG.PART: 67308S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed frozen tissues (e.g., pancreatic alpha-cells). Cytoplasmic background may be observed in human and monkey samples. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. |
| Background | Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| SKU | CSSPQ-67308S |
|---|---|
| Supplier Part Number | 67308S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |