Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Alexa Fluor 594 Conjugate)

Name: Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Alexa Fluor 594 Conjugate)
SKU: CSSPQ-70194S
Price: 465.00 USD

List Price $465.00 Your Price $465.00

Cell Signaling Beta-Catenin (D10a8) Xp Rabbit mAb (Alexa Fluor 594 Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-70194S

MFG.PART: 70194S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M R Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Immunofluorescence (Frozen): 1:50 - 1:400, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800, Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total β-catenin protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.
Background	β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).