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Cell Signaling Non-Phospho (Active) Beta-Catenin (Ser33/37/Thr41) (D13a1) Rabbit mAb (Alexa Fluor 647 Conjugate)
List Price $493.00 Your Price $493.00
Cell Signaling Non-Phospho (Active) Beta-Catenin (Ser33/37/Thr41) (D13a1) Rabbit mAb (Alexa Fluor 647 Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-60180S
MFG.PART: 60180S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33. This specificity data was derived from competition ELISA and DOT blot analysis using synthetic peptides. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein. |
| Background | β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814 is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and/or the canonical Wnt signaling pathway. |
| SKU | CSSPQ-60180S |
|---|---|
| Supplier Part Number | 60180S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |