Cell Signaling Phospho-Pras40 (Thr246) (C77d7) Rabbit mAb (BSA And Azide Free)

Name: Cell Signaling Phospho-Pras40 (Thr246) (C77d7) Rabbit mAb (BSA And Azide Free)
SKU: CSSPQ-60202SF
Price: 898.00 USD

List Price $898.00 Your Price $898.00

Cell Signaling Phospho-Pras40 (Thr246) (C77d7) Rabbit mAb (BSA And Azide Free) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-60202SF

MFG.PART: 60202SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µg
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	40
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #2997
Specificity/Sensitivity	Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40.
Background	Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).