Cell Signaling Lat (E3u6j) Xp ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)

Name: Cell Signaling Lat (E3u6j) Xp ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)
SKU: CSSPQ-91618S
Price: 465.00 USD

List Price $465.00 Your Price $465.00

Cell Signaling Lat (E3u6j) Xp ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - CSSPQ (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSSPQ-91618S

MFG.PART: 91618S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	LAT (E3U6J) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total LAT protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu88 of human LAT protein.
Background	LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).