We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Cell Signaling Granzyme K (E6z6o) Rabbit mAb
List Price $376.00 Your Price $376.00
Cell Signaling Granzyme K (E6z6o) Rabbit mAb - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-98946S
MFG.PART: 98946S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 17-27 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:400 - 1:800} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Granzyme K (E6Z6O) Rabbit mAb recognizes endogenous levels of total Granzyme K protein. This antibody does not cross-react with human Granzyme A, B, H, or M proteins. This antibody cross-reacts with a 300 kDa protein of unknown identity in some cell extracts. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human Granzyme K protein. |
| Background | Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).Granzyme K has tryptase-like activity with a preference for cleaving its specific substrates after basic residues, arginine or lysine (4-7). Granzyme K is expressed by NK T cells, cytotoxic T cells, and NK cells (8,9). After perforin-mediated entry into a target cell, Granzyme K induces apoptosis via generation of reactive oxygen species, DNA damage, and BID-dependent mitochondrial damage (10-12). |
| SKU | CSSPQ-98946S |
|---|---|
| Supplier Part Number | 98946S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |