We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Cell Signaling Phospho-Her2/Erbb2 (Tyr1221/1222) (6b12) Rabbit mAb (BSA And Azide Free)
List Price
$898.00
Your Price
$898.00
Cell Signaling Phospho-Her2/Erbb2 (Tyr1221/1222) (6b12) Rabbit mAb (BSA And Azide Free) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSSPQ-91415SF
MFG.PART:
91415SF
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µg |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 185 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {} |
| Storage | Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #2243 |
| Specificity/Sensitivity | Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb (BSA and Azide Free) detects endogenous levels of ErbB2 only when phosphorylated at tyrosines 1221/1222. The antibody does not detect other activated Erb family members or other tyrosine-phosphorylated proteins. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein. |
| Background | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| SKU | CSSPQ-91415SF |
|---|---|
| Supplier Part Number | 91415SF |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |