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Cell Signaling Mcm7 (D10a11) Xp ® Rabbit mAb (BSA And Azide Free)
List Price $865.00 Your Price $865.00
Cell Signaling Mcm7 (D10a11) Xp ® Rabbit mAb (BSA And Azide Free) - CSSPQ (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSSPQ-58963SF
MFG.PART: 58963SF
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µg |
| Reactivity | H M R Hm Mk Dg |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {} |
| Storage | Supplied in 1X PBS, BSA and Azide Free.For standard formulation of this product see product #3735 |
| Specificity/Sensitivity | MCM7 (D10A11) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of total MCM7 protein. Western blot analysis and immunofluorescent data indicate that the antibody is more reactive to primate than rodent proteins. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Dog |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MCM7. |
| Background | The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10). |
| SKU | CSSPQ-58963SF |
|---|---|
| Supplier Part Number | 58963SF |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSSPQ |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |