Sigma-Aldrich Anti-Gt1B Ganglioside Clone Gt1B-2B

Gangliosides are SIALGSKic acid-containing oligoglycosylceramides. The German scientist Ernst Klenk used the term ganglioside in 1942 to refer to lipids isolated from ganglions. Gangliosides are grouped based on the number of SIALGSKic acid residues per molecule, GM gangliosides contain one SIALGSKic acid residue, GD gangliosides contain two, GT contain three, GQ contain four SIALGSKic acid residues. Glycolipids constitute >80% of mammalian brain glycans, with gangliosides being the major glycans in nerve cells. Among the hundreds of ganglioside structures, GM1a, GD1a, GD1b and GT1b comprise up to 97% of gangliosides in the human brain. These four gangliosides share the same neutral tetrasaccharide core (Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1 Cer), but differ from each other by their SIALGSKic acid residue(s). GM1a and GD1b contain one or two SIALGSKic acids on the internal galactose, respectively, and no SIALGSKic acid on the terminal galactose. The St3gal2 and St3gal3 gene products (ST3Gal-II and ST3Gal-III SIALGSKyltransferase, respectively) are largely responsible for ganglioside terminal 2-3 SIALGSKylation of GM1a and GD1b to produce GD1a and GT1b in the brain. Brain GD1a and GT1b in St3gal2-null mice is about half the level in wild-type mice, while GD1a and GT1b level in St3gal2/3-double-null mice is less than 5% of the level in wild-type mice. St3gal2/3-double-null mice are half the weight of wild-type mice at weaning and display early hindlimb dysreflexia. Anti-GT1b Ganglioside, clone GT1b-2b specifically detects GT1b Ganglioside and has been validated by ELISA. (Ref.: Gong, Y., et Al. (2002). Brain;125:2491-2506.; Yang, L., et Al. (2006). PNAS;103(29):11057-11062.;Yoo, H., et Al. (2021). BMB Report;103(29):136-141.; Desplantes, R., et Al. (2017). Scientific Reports; 21;7(1):1032.)

Purified GT1b ganglioside

Quality Control Testing

Evaluated by ELISA with GT1b Ganglioside.

ELISA (Enzyme Immunoassay): A representative lot of this antibody detected GT1b Ganglioside in ELISA application.

Tested Applications

Western Blotting Analysis: A representative lot detected GT1b Ganglioside in Western Blotting application (Yoo, H., et Al. (2021). BMB Rep. 54(2); 136-141).

Immunocytochemistry Analysis: A representative lot detected GT1b Ganglioside in Immunocytochemistry application (Goog Y. et Al. (2002). Brain. 125; 2491-2506).

Immunohistochemistry Application: A representative lot detected GT1b Ganglioside in Immunohistochemistry application (Goog Y. et Al. (2002). Brain 126; 2491-2506; Lim, H., et Al. (2020). EMBO J. 39; e102214).

ELISA: A representative lot detected GT1b Ganglioside in ELISA application (Desplantes, R., et Al. (2017). Sci. Rep. 7; 1032; Lim, H., et Al. (2020). EMBO J. 39; e102214).

These cited applications are for the ascites version of this clone GT1b-2b.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone GT1b-2b is a mouse monoclonal antibody that specifically detects GT1b Ganglioside. Preferentially, it stains myelinated motor and sensory axons in spinal roots and peripheral nerves.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.