Sigma-Aldrich Anti-Sarm1 Clone 10G2 100 Mig

NAD(+) hydrolase SARM1 (UniProt: Q6PDS3; also known as EC:3.2.2.6, NADase SARM1, NADP(+) hydrolase SARM1, Sterile alpha and TIR motif-containing protein 1) is encoded by the Sarm1 (also known as Kiaa0524) gene (Gene ID: 237868) in murine species. The encoded protein belongs to the MyD88 (myeloid differentiation primary response gene 88) family of adaptor proteins. SARM1 is a critical protein involved in axonal degeneration, particularly in response to injury and neurotoxic insults. It is widely expressed in the brain and neurons. It is also reported to be expressed in photoreceptor cells of the neural retina. SARM1 predominantly localizes in the cytoplasm and, studies show its localization in mitochondria, axons and dendrites. The protein is synthesized with a transit peptide (aa 1-27) and mature protein contains 8 armadillo repeat (ARM) regions, SAM (sterile motif) domains and a TIR (toll-interleukin receptor) domain. The TIR domain facilitates its activation through dimerization. This activation leads to the hydrolysis of NAD+, a process essential for its function as an axon degeneration executor. SARM1 has been implicated in inflammatory responses particularly in the central nervous system. It can influence the activation of microglia, the immune cells of the brain, and is involved in the regulation of neuroinflammatory processes. This suggests its potential role in diseases characterized by chronic inflammation, such as multiple sclerosis and Alzheimer′s disease. This mouse monoclonal 10G2 antibody has been validated and cited for Western blotting, Immunohistochemistry, Immunoflourescence and Immnuoprecipitation. Studies conducted with this antibody suggests that SARM1 also plays multiple roles in neuronal morphogenesis and, it is specifically required for proper initiation and elongation of dendrites, axonal outgrowth, and neuronal polarization. These functions likely involve Sarm1-mediated regulation of microtubule stability, as Sarm1 influenced tubulin acetylation. (Ref.: Chen, C-Y., et Al. (2011). J Cell Biol. 193(4):769-84; Panneerselvam, P., et Al. (2013). Cell Death Differ. 20(3):478-89; Lin, C-W., et Al. (2014). Innate Immun. 20(2):161-72; Gilley, J., et Al. (2015). Cell Rep. 10(12):1974-81; Li, Y., et Al. (2022). J Cell Biol. 221(2):e202106080).

MBP-tagged, recombinant fragment corresponding to 410 amino acids from N-terminal region of mouse SARM1.

Quality Control Testing

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis (WB): A 1:500 dilution from a representative lot detected SARM1 in Neuro-2a cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected SARM1 in Western Blotting applications (Chen, C-Y., et Al. (2011). J Cell Biol. 193(4):769-84; Panneerselvam, P., et Al. (2013). Cell Death Differ. 20(3):478-89; Gilley, J., et Al. (2015). Cell Rep. 10(12):1974-81; Li, Y., et Al. (2022). J Cell Biol. 221(2):e202106080).

Immunocytochemistry Analysis: A representative lot detected SARM1 in Immunocytochemistry application (Chen, C-Y., et Al. (2011). J Cell Biol. 193(4):769-84).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SARM1 in Immunoprecipitation application (Chen, C-Y., et Al. (2011). J Cell Biol. 193(4):769-84).

Immunofluorescence Analysis: A representative lot detected SARM1 in Immunofluorescence application (Lin, C-W., et Al. (2014). Innate Immun. 20(2):161-72).

Immunohistochemistry Applications: A representative lot detected SARM1 in Immunohistochemistry applications (Chen, C-Y., et Al. (2011). J Cell Biol. 193(4):769-84; Lin, C-W., et Al. (2014). Innate Immun. 20(2):161-72).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 10G2 is a mouse monoclonal antibody that specifically detects SARM1. It targets an epitope within 410 amino acids from the N-terminal region.

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.