Sigma-Aldrich Anti-Fibromodulin Clone 636B12

Fibromodulin (UniProt: Q06828; also known as FM, Collagen-binding 59 kDa protein Keratan sulfate proteoglycan fibromodulin (KSPG fibromodulin)) is encoded by the FMOD (also known as FM, SLRR2E) gene (Gene ID: 2331) in human. Fibromodulin is a class II small leucine-rich proteoglycan (SLRP) that plays a significant role in the extracellular matrix (ECM). Structurally, fibromodulin consists of leucine-rich repeat (LRR) motifs that are crucial for its interaction with collagen fibrils. It binds to ECM structural components and regulates collagen cross-linking, packing, assembly, and fibril architecture, impacting tissue integrity and repair, influencing the mechanical properties of tissues such as tendons, cartilage, and skin. It also plays a role in cell proliferation, differentiation, adhesion, migration, invasion, and metastasis by interacting with cytokines and growth factors, particularly with TGFβ superfamily members. Fibromodulin is predominantly expressed in connective tissues, including tendons, ligaments, and cartilage. Fibromodulin has been implicated in disease such as osteoarthritis, where altered expression or function of fibromodulin can lead to impaired collagen organization and joint degradation. Fibromodulin is a prevalent stromal component in soft tissue sarcomas and has been implicated in fibrosis and certain cancers, where it may influence tumor progression and metastasis by affecting the ECM composition and cell behavior. (Ref.: Zheng, Z., et Al., J of Dent Res. 102(2), 125 134; Zhao, F., et Al., (2023) Front. Immunol. Sec. Molecular Innate Immunity, 14, 10.3389/fimmu.2023.1191787; Benassi, M.S., et Al. (2009). J Orthop Res. 27(1):135-40).

Purified human fibromodulin from human articular cartilage.

Quality Control Testing

Evaluated by Western Blotting with hFc-tagged, recombinant human Fibromodulin.

Western Blotting Analysis: A 1:500 dilution of this antibody detected hFc-tagged, recombinant human Fibromodulin.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Fibromodulin in Immunohistochemistry application (Benassi, M.S., et Al. (2009). J Orthop Res. 27(1):135-40).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 636B12 is a mouse monoclonal antibody that detects Fibromodulin.

Purified mouse monoclonal antibody IgM in PBS without preservatives.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.