Sigma-Aldrich Anti-Mucin 5Ac Clone 2K18 Zoomab Rb

Mucin-5AC (UniProt: P98088; also known as MUC-5AC, Gastric mucin, Major airway glycoprotein, Mucin-5 subtype AC, tracheobronchial, Tracheobronchial mucin (TBM)) is encoded by the MUC5AC (also known as MUC5) gene (Gene ID: 4586) in human. Mucin-5AC is a high molecular weight glycoprotein belonging to the mucin family, characterized by extensive glycosylation and tandem repeat domains rich in serine and threonine residues. Mucin-5AC forms polymeric gels through disulfide-mediated multimerization, contributing to its viscoelastic properties. Mucin-5AC plays a critical role in protecting epithelial surfaces by forming a physical barrier against pathogens and environmental insults, while also participating in immune modulation by interacting with cell surface receptors and microbial adhesins. It is predominantly expressed in goblet cells of the respiratory and gastrointestinal tracts, with localization to the mucus layer covering epithelial surfaces. Mucin-5AC interacts with proteins such as P300, which regulates its expression via histone acetylation, and other mucins like MUC5B to maintain mucus homeostasis. Dysregulation of MUC5AC expression is implicated in various diseases, including asthma, chronic obstructive pulmonary disease (COPD), and gastric cancers, where excessive secretion contributes to mucus hyperplasia and impaired clearance. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 20 amino acids from the internal of human Mucin 5AC.

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.


Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Mucin 5AC in A549 cell lysate.


Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Mucin 5AC in human stomach tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Mucin 5AC in human stomach tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound with Mucin 5AC peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

ELISA Analysis: A representative lot of this antibody detected Mucin 5AC protein in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 2K18 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Mucin 5AC. It targets an epitope within 20 amino acids from the internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.