Sigma-Aldrich Anti-Sgk1 Clone 2N11 Zoomab Rb Mono

Serine/threonine-protein kinase Sgk1 (UniProt: O00141; also known as Serum/glucocorticoid-regulated kinase 1, EC:2.7.11.1) is encoded by the SGK1 (also known as SGK) gene (Gene ID: 6446) in human. SGK1 is a serine/threonine kinase that is induced in response to a variety of stimuli, including serum, osmotic shock, FSH, glucocorticoids, and mineralocorticoids. It is expressed in most tissues with highest levels observed in the pancreas, followed by placenta, kidney, and lung. Its protein kinase domain is localized in amino acids 98-355. The subcellular localization of SGK1 is controlled by the cell cycle, as well as by exposure to specific hormones and environmental stress stimuli. In proliferating cells, it shuttles between the nucleus and cytoplasm in synchrony with the cell cycle, and in serum/growth factor-stimulated cells it resides in the nucleus. SGK1 is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. It is also involved in the regulation of renal sodium retention and potassium elimination. SGK1 activity is regulated by phosphorylation and is activated by phosphorylation on Ser422 by mTORC2. This phosphorylation transforms it into a substrate for PDPK1, which phosphorylates it on Thr256. Phosphorylation on Ser397 and Ser401 are also considered to be essential for its activity. High levels of expression of SGK1 gene may contribute to conditions such as hypertension and diabetic nephropathy. SGK1 has been reported to be up-regulated in non-small cell lung cancer (NSCLC) and its over-expression promotes the growth and migration of NSCLC cells via GSK-3 beta phosphorylation and activation of beta-catenin/TCF signaling. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Xiaobo, Y., et Al. (2016) Gene 576, 339-346).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human SGK1.

Quality Control Testing

Evaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SGK1 in SK-MEL-28 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SGK1 in lysate from mouse heart tissue and a 1:10,000 dilution from a representative lot detected SGK1 in lysates from human heart tissue and rat heart tissue.

Affinity Binding Assay: A representative lot of this antibody bound SGK1 peptide with at least one hundred thousand-fold (100,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SGK1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SGK1 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2N11 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects SGK1. It targets an epitope within 17 amino acids from the Internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

0.3 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.