Sigma-Aldrich Anti-Xrcc1 Clone 3G7 Zoomab Rabbit

DNA repair protein XRCC1 (UniProt: P18887; also known as X-ray repair cross-complementing protein 1) is encoded by the XRCC1 gene (Gene ID:7515) in human. XRCC1 is a scaffold protein that is involved in DNA single-strand break repairs by mediating the assembly of DNA break repair protein complexes. Upon detection of DNA damage, it moves from the nucleoli to the global nuclear chromatin. It specifically interacts with nicked and gapped DNA and acts as a nick sensor capable of interacting with a variety of other proteins involved in repair, including DNA polymerases and DNA ligases. XRCC1 is expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level). It contains three distinct domains: the N-terminal domain, and two independent BRCT domains (aa 315-403 and 539-629), connected by two intervening unstructured linkers that serve as critical interacting platforms for various repair factors. The first BRCT domain is reported to interact with PARP1, APE1, MPG, NTH1 and NEIL1 and the second BRCT domain interacts with DNA ligase III. It is shown to negatively regulate ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. XRCC1 may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mok, MCY., et Al. (2019). Sci. Rep. 9; Article 3095; Caldecott, KW., et Al. (1995). Nucl. Acids Res. 23(3); 4836-4843).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human XRCC1.

Quality Control Testing

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XRCC1 in HT-29 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected XRCC1 in lysates from RT4 cells, mg-63 cells, and human hippocampus tissue.

Affinity Binding Assay: A representative lot of this antibody bound XRCC1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected XRCC1 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 3G7 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects XRCC1. It targets an epitope within 21 amino acids from the internal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.