Sigma-Aldrich Anti-Ftl Clone 2F12 Zoomab Rb Mono

Ferritin light chain (UniProt: P02792; also known as Ferritin L subunit) is encoded by the FTL gene (Gene ID: 2512) in human. The Ferritin light chain (FTL) is a subunit of the ferritin protein complex, which is a highly conserved iron-storage molecule composed of 24 polypeptide subunits. FTL contributes to the formation of the hollow spherical protein cage, with external and internal diameters of approximately 12 nm and 8 nm, respectively. FTL plays a critical role in iron sequestration and storage, promoting the nucleation and stabilization of iron(III) within the ferritin core. Unlike the heavy chain subunit, FTL lacks ferroxidase activity but is essential for maintaining the structural integrity of the ferritin complex. FTL is expressed ubiquitously across tissues, with elevated levels in the liver, spleen, and brain, and is localized primarily in the cytoplasm. It interacts with various proteins, including NCOA4, which mediates ferritinophagy a specialized autophagic pathway that releases stored iron for cellular use. Dysregulation of FTL has been implicated in several diseases, such as hereditary ferritinopathy, a neurodegenerative condition characterized by abnormal ferritin and iron accumulation, and neurodegenerative disorders associated with iron dysregulation. FTL mutations can lead to altered iron storage capacity and enhanced aggregation, contributing to cellular toxicity. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 26 amino acids from the C-terminal region of human FTL.

Quality Control Testing

Evaluated by Western Blotting in K-562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FTL in K-562 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected FTL in human liver tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected FTL in human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound with FTL peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Clone 2F12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects FTL. It targets an epitope within 26 amino acids from the C-terminal region.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

0.3 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.