Sigma-Aldrich Anti-Nogo Receptor Cl 1E18 Zoomab

Reticulon-4 receptor (UniProt: Q9BZR6; also known as Nogo receptor (NgR), Nogo-66 receptor) is encoded by the RTN4R (also known as NOGOR, UNQ330/PRO526) gene (Gene ID: 65078) in human. The Nogo receptor (NgR) is a glycophosphatidylinositol (GPI)-anchored cell-surface protein that plays a significant role in axonal growth inhibition and neural plasticity. It is characterized by eight leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal disulfide-containing cap domains, which contribute to its stability and ligand binding specificity. These LRRs are crucial for interactions with myelin-associated inhibitors such as Nogo-A, oligodendrocyte myelin glycoprotein (OMgp), and myelin-associated glycoprotein (MAG), which mediate the suppression of axon regeneration in the central nervous system (CNS). The receptor also contains a C-terminal stalk region and is anchored to the cell membrane via a GPI linkage, which positions it optimally for extracellular ligand interactions and signaling. The Nogo receptor is critical regulator of axonal growth and neural plasticity, primarily studied in the context of neuroregeneration and central nervous system (CNS) development. The Nogo receptor interacts with ligands such as Nogo-A, a myelin-associated inhibitor, to inhibit axon sprouting and regeneration, restricting neuroaxonal plasticity in adult mammals. This receptor is predominantly expressed in neurons and plays a key role in limiting recovery after CNS injuries, such as spinal cord injury (SCI). Recent studies have highlighted its role in modulating neuroinflammation, mitochondrial function during conditions like multiple sclerosis, and its impact on amyloid-β (Aβ) production in Alzheimer s disease pathogenesis. Additionally, NgR signaling has been implicated in regulating neurite outgrowth, precursor migration, and branching during nervous system development. The Nogo receptor also plays non-neuronal roles, such as regulating macrophage polarization and recruitment during inflammatory responses, further broadening its significance in neurodegenerative diseases and systemic inflammation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dave, B.P., et Al., (2023), Biochem Pharmacol. 210,115461).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Nogo Receptor.

Quality Control Testing

Evaluated by Western Blotting in Neuro-2a cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Nogo Receptor in Neuro-2a cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nogo Receptor in A549 cell lysate.

Enzyme Immunoassay: A representative lot of this antibody detected Nogo Receptor protein in ELISA application.

Affinity Binding Assay: A representative lot of this antibody bound with Nogo Receptor peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1E18 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Nogo Receptor. It targets an epitope within 20 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.